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Electrophysiology of the isolated and perfused midgut of adult female yellow fever mosquitoes ( Aedes aegypti )
Author(s) -
Onken Horst,
Fuks Yolana,
Valencia Melanie,
Moffett Stacia B,
Moffett David F
Publication year - 2010
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.24.1_supplement.813.8
Subject(s) - midgut , pipette , aedes aegypti , perfusion , theophylline , lumen (anatomy) , hindgut , dinitrophenol , biology , anatomy , malpighian tubule system , chemistry , medicine , endocrinology , microbiology and biotechnology , ecology , larva
The midgut of adult, female mosquitoes receives the blood meal. Salt and water are rapidly absorbed and secreted by the Malpighian tubules. Later, nutrients are digested and absorbed. Transport in the midgut of adult mosquitoes has never been studied with isolated midguts. Isolated midguts were transferred into a bath with mosquito saline and mounted on perfusion pipettes connected to a syringe pump. The transepithelial voltage (V te ) was measured. Mounting midguts with the posterior end on the pipette allows continuous perfusion, and a small, lumen negative V te was observed. Addition of theophylline (10 mmol/l, increasing intracellular cAMP concentrations) to the bathing solution resulted in a small, lumen positive V te . Because a valve is located between midgut and hindgut, the posterior midgut is inflated when the anterior part connects to the perfusion pipette. When inflated with mosquito saline the initial, lumen negative V te and the lumen positive V te after addition of theophylline were markedly higher, suggesting that stretch is a stimulator for the ion transport characteristics reflected in V te . V te is inhibited with dinitrophenol, indicating that it reflects active ion transport. Financial support by the NIH (1R01AI063463‐01A2) and by a Wagner College Faculty Research Grant is gratefully acknowledged.