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Novel Methods to Investigate Multi‐fiber Nerve Recordings
Author(s) -
Burgess Don Edward,
Verberne Anthony J.M.,
Sartor Daniela M.
Publication year - 2010
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.24.1_supplement.808.12
Subject(s) - splanchnic nerves , rostral ventrolateral medulla , microneurography , splanchnic , vasomotor , bursting , chemistry , medicine , sympathetic nerve , neuroscience , endocrinology , biology , blood flow , central nervous system , baroreflex , blood pressure , stimulation , medulla oblongata , heart rate
We developed novel methods to investigate responses of neuronal populations within a multi‐fiber sympathetic nerve recording. We applied these methods to analyze the relationship between barosensitive RVLM medullospinal neurons marked by their response to systemic administration of the gastrointestinal hormone cholecystokinin (CCK) and splanchnic and lumbar sympathetic nerve activity (SNA). In addition to the standard analysis of Spike‐Triggered Averaging (STA) of SNA, we used Spike2 software to identify spike‐like structures within the sympathetic nerve recording (SND). We then calculated cross‐correlograms between apparent “spikes” in SND and RVLM neuronal discharges. Also, we calculated a STA of RVLM mean frequency triggered by the identified “spikes”. These novel analyses yielded consistent results with the standard STA of SNA and detected sympathetic nerve discharges driven only by the fast pathway. We find statistically significant peaks (p<0.001) in the cross‐correlogram between CCK inhibited neurons and “spikes” driven by the fast pathway (n=5). The time‐delay between RVLM neuronal discharges and the fast burst of SNA in splanchnic nerves is 52 +/− 2ms. The corresponding delay for lumbar nerves is 78+/− 4ms. These novel analyses may serve as a tool to study differential behavior of populations of neurons that drive sympathetic vasomotor nerve activity. Supported by R01 NS39774, HL091440