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Strenuous Exercise Enhances Thrombin Generation Induced by Platelet‐derived Microparticles
Author(s) -
Wang JongShyan,
Chen YuWen
Publication year - 2010
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.24.1_supplement.806.2
Subject(s) - thrombin generation , platelet , phosphatidylserine , chemistry , thrombin , tissue factor , flow cytometry , endocrinology , medicine , platelet activation , coagulation , immunology , biochemistry , phospholipid , membrane
This investigation elucidates how strenuous exercise affects platelet‐derived microparticles (PDMP) with procoagulant factors and PDMP‐mediated thrombin generation (TG) under pathological high (HS, 100 dyne/cm 2 ) shear stress by closely examining 24 sedentary men who exercised strenuously (up to VO 2max ) on a bicycle ergometer. The PDMP/platelets with procoagulant factors and the dynamic TG in plasma were measured using two‐color flow cytometry and a calibrated, automated thrombinography, respectively. The results showed that HS increased the levels of total PDMP and the PDMP binding procoagulant factor V/Va (FV/Va) or VIII (FVIII), expressing tissue factor (TF), or exposing phosphatidylserine (PS), which were accompanied by elevated the height of thrombin peak and increased the rate of TG in PDMP‐rich plasma. Moreover, the HS simultaneously enhanced the binding of FV/Va or FVIII to platelets and the exposure of PS on platelets. Under the HS condition, strenuous exercise increased the counts of FV/Va‐/FVIII‐/TF‐rich PDMP and PS‐exposed PDMP and augmented PDMP‐promoted peak height and rate of TG, whereas the ability to binding of FV/Va or FVIII to platelets and the extent of PS exposure on platelets were enhanced following this exercise. Therefore, we conclude that strenuous exercise enhances shear‐induced TG by increased the release of procoagulant‐rich microparticles from platelets.