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Effect of 3, 6, and 12 wks Voluntary Exercise on Renal Endothelial Enzymes
Author(s) -
Moningka Natasha,
Ahlgren Joslyn,
Hayward Linda,
Baylis Chris
Publication year - 2010
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.24.1_supplement.804.6
Subject(s) - enos , endocrinology , medicine , sed , nitric oxide synthase , endothelial nitric oxide synthase , superoxide dismutase , nitric oxide , chemistry , zoology , oxidative stress , biology
Exercise (EX) markedly reduces renal blood flow (RBF) which could also reduce the shear stress induction of the nitric oxide (NO) generating enzyme, endothelial NO synthase (eNOS), and the antioxidant, extracellular superoxide dismutase (EC SOD). The duration of EX training may also play a role. Therefore, we investigated the impact of EX duration on eNOS and EC SOD abundance in the kidney cortex (KC). Male Sprague Dawley rats were given 24 hr access to voluntary wheel running for 3, 6, or 12 wks, or remained sedentary (SED). Running activity was low intensity and comparable during the first 3 wks for all groups in that there was a steady increase in avg. total distance run from week 1, 2, and 3 (representative activities: 0.9±0.31, 2.1±0.68, and 4.0±1.3 km/day, respectively), and remained ~3–5 km/day over the next 9 wks. KC abundance of eNOS and EC SOD were unchanged with 3 wks EX, but significantly decreased by 6 and 12 wks ( Table; *p<0.05 vs. SED). The duration/intensity of EX is important since during the first 3 wks of voluntary EX, where running activity was increasing, there were no changes in KC eNOS or EC SOD. By 4 wks, running reached a maximum value and plateaued (~5 km/day), and by 6 and 12 wks there were significant reductions in KC eNOS and EC SOD. The falls in eNOS and EC SOD are predictable based on the presumed reductions in RBF. Our data suggest that the duration of EX plays a critical role in dictating the kidney's response of eNOS and EC SOD abundance to EX. Further study is required to determine the long term impact of EX on kidney function and structure in health and disease.KC eNOS KC EC SODn Arbitrary Units Arbitrary Units3 wkSED 3 1.2±0.2 3.2±0.9 EX 3 1.2±1.1 6.7±3.76 wkSED 8 25±1.7 21±3.2 EX 12 18±2.8* 17±5.3*12 wkSED 6 64±2.6 13±1.0 EX 6 56±2.2* 8.0±0.7*