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Pulmonary vein endothelial cells (PVECs) exhibit characteristics of multiple lung endothelial cell phenotypes
Author(s) -
Creighton Judy,
Buford Anna,
Ayers Linn,
Stevens Troy
Publication year - 2010
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.24.1_supplement.797.13
Subject(s) - endothelial stem cell , cell sorting , lung , phenotype , flow cytometry , endothelium , pulmonary artery , pulmonary vein , biology , doubling time , pathology , microbiology and biotechnology , immunology , cell , in vitro , chemistry , medicine , endocrinology , biochemistry , ablation , gene , genetics
Endothelial cells lining the lungs' pulmonary artery, capillary, and venous blood vessels exhibit vastly different morphologies and functions. We have previously shown that segment specific attributes of pulmonary artery endothelial cells (PAECs) and pulmonary microvascular endothelial cells (PMVECs) are retained in culture. However, fundamental information regarding the characteristics of endothelial cells obtained from the pulmonary vein is lacking. PMVECs are known to possess a higher growth rate than PAECs. We determined that PVECs have a growth rate (doubling time) that is only slightly higher than PAECs (29hrs vs. 33 hrs). PMVECs develop a tighter barrier than do PAECs (2000 ohms vs. 1100 ohms, respectively). Interestingly, PVEC barrier resistance is only slightly less than PMVECs (1800 ohms). All three phenotypes express typical endothelial markers, yet display distinct lectin binding patterns as determined by Fluorescence Activated Cell Sorting (FACS) and immunocytochemical analysis. Taken together these data demonstrate functional heterogeneity among lung endothelial cell phenotypes including those derived from the pulmonary vein. Supported by HL66299.