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Trefoil factor‐3 is down regulated while CYP24a1 is increased in the ageing rat kidney
Author(s) -
Hultström Michael,
Iversen Bjarne M.
Publication year - 2010
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.24.1_supplement.791.5
Subject(s) - ageing , kidney , gene expression , cyp24a1 , biology , trefoil , medicine , endocrinology , vitamin d and neurology , gene , calcitriol receptor , biochemistry , agronomy
The large scale expressional changes in ageing kidneys are not well described. In the present experiment we compared the genome wide expression of mRNAs in both kidneys from five 90 week old rats against five 10 week old rats. The expression from left and right kidneys were compared to determine if the samples should be used as independent samples or as replicates. No significant differences in gene expression were found between left and right kidneys. They showed a homogeneity almost comparable to technical replicates. Pooling results from left and right kidneys produced 20 genes showing a larger than two‐fold change. The most down regulated gene was Trefoil factor‐3, which has been shown to be lower in ageing kidneys previously. The most upregulated gene was cytochrome P450‐24a1, known to hydroxylate Vitamin D3. In conclusion, the left and right kidney cortex show very similar expression patterns, making them very useful for paired experiments but not appropriate as independent samples. Vitamin D3 metabolism is likely changed in the ageing kidney as a result of increased CYP24a1 expression.