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Fluid Resuscitation Physiological Responses in Hemorrhaged Rats
Author(s) -
Doursout MarieFrancoise Joelle,
Philip Asher,
Liang Yang Yan,
Deng Xiyun,
Conyers Jodie,
Holcomb John
Publication year - 2010
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.24.1_supplement.788.9
Subject(s) - resuscitation , medicine , blood pressure , peroxynitrite , xanthine oxidase , shock (circulatory) , nitrotyrosine , anesthesia , nitric oxide , endothelial dysfunction , mean arterial pressure , chemistry , heart rate , nitric oxide synthase , biochemistry , superoxide , enzyme
Hemorrhagic hypotension leads to a well‐characterized sequence of physiological events. This includes decreases in cardiac output and blood pressure, the release of vasoconstrictors, hyporeactivity and vascular decompensation leading to the production of reactive oxygen species (ROS) and nitrogen oxide (peroxynitrite). ROS caused injuries to the vascular endothelium resulting in endothelial dysfunction leading to multiple organ failure in hemorrhagic shock (HS). Our study assessed the production of nitrate, ROS and peroxynitrite in hemorrhaged rats with and without resuscitation. Rats were hemorrhaged at a rate of 2ml/kg over 10 min until mean arterial blood pressure dropped to 30 mmHg. One hour following HS rats were resuscitated with either Fresh Frozen Plasma (FFP; Day 0/Day 5) or Lactated Ringer (LR). Additional rats included HS alone and sham control. Our data demonstrated that FFP Day 0 increased nitrate production, supporting a vasodilatation in vascular beds, as compared to FFP Day 5; LR and HS alone. Our data shown that Xanthine Oxidase, an enzyme that generates ROS, was predominant in HS alone and in LR‐resuscitated rats whereas rats resuscitated with FFP Day 0 decreased ROS production. Finally, resuscitation with FFP at Day 0 as compared to FFP Day 5 and LR decreased nitrotyrosine production. Our results indicate that FFP Day 0 improved resuscitation as compared to FFP Day 5 and LR in rats subjected to HS.