Premium
Chronic ingestion of a western diet alters O‐linked‐β‐N‐acetylglucosamine (O‐GlcNAc) protein modification in the heart
Author(s) -
Medford Heidi M,
Chatham John C,
Marsh Susan A
Publication year - 2010
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.24.1_supplement.787.9
Subject(s) - glycation , medicine , endocrinology , endoplasmic reticulum , threonine , diabetes mellitus , ingestion , weaning , carbohydrate , chemistry , fibrosis , serine , biology , phosphorylation , biochemistry
We recently demonstrated that myocardial fibrosis was increased in young rats fed a diet high in saturated fat and simple carbohydrate (“western” diet, WD) for 2 weeks, which was sustained at 14 weeks. To determine the chronic effects of such a diet, we fed male Sprague‐Dawley rats either control diet (n=9) or WD (n=10) for 12 months. Blood chemistry analyses exhibited numerous abnormalities in the WD group, indicative of liver, kidney, and metabolic dysfunction. Surprisingly, left ventricular function was not different between diets. In myocardial tissue, there were no differences between diets in fibronectin, endoplasmic reticulum stress (GRP78, phosphorylated IRE1), or advanced glycation end‐products. Increased levels of O‐linked‐β‐N‐acetylglucosamine (O‐GlcNAc) on serine and threonine residues has been linked to metabolic disease, and is elevated in the heart in response to diabetes. We found that O‐GlcNAc levels increased in some but not all protein bands in the WD group; at least one protein band exhibited decreased intensity with WD. These data suggest that aging attenuates the differences in fibrosis observed between diets in younger animals, but that chronic ingestion of a WD alters the pattern of O‐GlcNAc protein modification in the heart in the absence of overt cellular stress.