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Circulating angiogenic factors in a rat model of preeclampsia
Author(s) -
Agunanne Enoch,
Uddin Mohammad N,
Horvat Darijana,
Puschett Jules
Publication year - 2010
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.24.1_supplement.786.11
Subject(s) - preeclampsia , gestation , pregnancy , medicine , saline , endocrinology , placental growth factor , pathogenesis , antagonist , andrology , biology , receptor , genetics
Objective We studied the angiogenic factors in our rat model of preeclampsia (PE) in which marinobufagenin (MBG, a bufodienolide) plays an important role. Methods Animals were grouped into: normal, non‐ pregnant (C); normal pregnant (NP); NP injected weekly with DOCA intraperitoneally (IP) and whose drinking water was replaced with 0.9% saline (PDS); NP given daily injections of MBG (0.765 μg/100g) once pregnancy was established; PDS given daily injections of resibufogenin [RBG, an antagonist of MBG (30 μg/kg/day)] IP from day 4 of pregnancy (PDSR). The angiogenic factors were measured on 3–5, 7–10 & 17–20 days of gestation. We have previously shown that MBG levels are already elevated in this model at the 3–5 day time period and remain elevated throughout gestation in PDS rats. Summary of Results At 3–5 days of pregnancy, there was no angiogenic imbalance. At the 7–10 day period, plasma PlGF was greater in the NP than in the PDS. The sFlt‐1/PlGF as well as the placental Flt‐1 and Flt‐1/PlGF were greater in the PDS rats than in NP (p<0.05). These findings were also present at the 17–20 day time point. The levels of these factors in PDSR were similar to those in NP. Conclusions Angiogenic imbalance contributes to the pathogenesis of the preeclamptic syndrome in our rat model. MBG elevation precedes this alteration in angiogenic factors. This is confirmed by prevention of this imbalance in this study by pretreatment with RBG. Scott, Sherwood/Brindley Foundation.