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Near‐Infrared Photobiomodulation Protects Against Diabetes‐Induced Glutathione Oxidation in White Gastrocnemius Muscle
Author(s) -
Wilson Robert William,
Gopalakrishnan Sandeep,
Eells Janis T,
Snyder Ann C
Publication year - 2010
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.24.1_supplement.783.11
Subject(s) - streptozotocin , glutathione , medicine , endocrinology , oxidative stress , chemistry , gastrocnemius muscle , diabetes mellitus , myoglobin , antioxidant , carbohydrate metabolism , skeletal muscle , biochemistry , enzyme
Diabetic hyperglycemia increases ROS and depletes glutathione (GSH). Near‐infrared (NIR) photobiomodulation (PBM) has been shown to protect against antioxidant depletion in animal models of chronic degenerative disease. We tested the hypothesis that NIR PBM would protect gastrocnemius muscle (G) from hyperglycemia‐induced oxidative stress (OxS) in streptozotocin (STZ)‐induced diabetes. Male Wistar rats were randomly assigned to 3 groups: Control (CON), STZ‐treated rats (STZ) and 670 nm NIR treated STZ rats (NIR). STZ (50 mg/kg, ip) was administered on day (d) 0. NIR‐treated rats were treated with NIR for 5 min (15 J/cm2) from d3 – d98. White (WG) and red (RG) sections of the gastrocnemius muscles were analyzed for total, reduced and oxidized GSH (GSHT, GSH R , GSSG). STZ‐induced hyperglycemia had no effect on the GSSG:GSH R ratio in RG. In contrast, STZ treatment profoundly increased the GSSG:GSH R ratio in WG. NIR treatment restored the GSSG:GSH R ratio in WG to control values, but had no effect on the RG ratio. The differential response of RG and WG to the hyperglycemia and NIR treatment may be due to differences in energy metabolism. WG has few mitochondria and increased susceptibility to hyperglycemia‐induced OxS. NIR treatment was protective in WG. In contrast, the abundance of mitochondria and myoglobin in RG increased resistance to STZ‐induced metabolic changes and reduced NIR treatment impact. Funded by UWM RGI