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Early‐staged electro‐cardiac conduction in larvae and embryonic zebrafish
Author(s) -
YU FEI,
Wolla Christopher David,
Chen Jaunian,
Hsiai Tzung
Publication year - 2010
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.24.1_supplement.781.2
Subject(s) - zebrafish , qrs complex , danio , embryonic heart , anatomy , biomedical engineering , biology , medicine , cardiology , embryonic stem cell , chemistry , biochemistry , gene
Zebrafish (Danio rerio) is an emerging model organism for pharmacological studies, tissue engineering, and genetically modified cardiomyocytes. However, early‐staged cardiac function in zebrafish has not been well understood. We proposed to monitor electrocardiography (ECG) in zebrafish at embryonic and larva stage. Larvae were sedated with 0.01% tricane methanesulfonate and placed in E3 medium (5 mM NaCl, 0.17 mM KCl, 0.4 mM CaCl2, and 0.16 mM MgSO4) under microscope. A 5μm micropipette filled with 300% Danieau solution were in direct contact with the larva heart for voltage signal recording, and a second micropipette electrode was connected to the ground. Signal processing and wavelet transform enhanced the signal‐to‐noise ratios. ECG signals displaying distinct P wave and QRS complex were acquired from 7 days post‐fertilization (dpf) to 42 dpf. Larva ECG patterns started to transform to the adult features at 35 dpf. When the larvae were treated with Amiodarone (1μM), a Class III anti‐arrhythmic potassium channel blocker, single‐peak QRS complex degenerated into multiple‐peak and wide QRS duration. Thus, we provide a unique in vivo zebrafish model to assess developmental‐staged cardiac conduction phenotype with implication for high throughput research. Research supported by NHLB 083015 and NHLBI 068689.