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Polycystin‐1 and ‐2 Dosage Regulates Pressure Sensing
Author(s) -
Duprat Fabrice,
Naeni Reza Sharif,
Folgering Joost H.A.,
Bichet Delphine,
Lauritzen Inger,
Malika Arhatte,
Jodar Martine,
Retailleau Kevin,
Loufrani Laurent,
Patel Amanda,
Peters Dorien J.M.,
Honoré Eric
Publication year - 2010
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.24.1_supplement.780.7
Subject(s) - autosomal dominant polycystic kidney disease , pkd1 , polycystic kidney disease , microbiology and biotechnology , trpv4 , transient receptor potential channel , cilium , biology , chemistry , kidney , endocrinology , receptor , genetics
Autosomal‐dominant polycystic kidney disease, the most frequent monogenic cause of kidney failure, is induced by mutations in the PKD1 or PKD2 genes, encoding polycystins TRPP1 and TRPP2, respectively. Polycystins are proposed to form a flow‐sensitive ion channel complex in the primary cilium of both epithelial and endothelial cells. However, how polycystins contribute to cellular mechanosensitivity remains obscure. Here, we show that TRPP2 inhibits stretch‐activated ion channels (SACs). This specific effect is reversed by coexpression with TRPP1, indicating that the TRPP1/TRPP2 ratio regulates pressure sensing. Moreover, deletion of TRPP1 in smooth muscle cells reduces SAC activity and the arterial myogenic tone. Inversely, depletion of TRPP2 in TRPP1‐deficient arteries rescues both SAC opening and the myogenic response. Finally, we show that TRPP2 interacts with filamin A and demonstrate that this actin crosslinking protein is critical for SAC regulation. This work uncovers a role for polycystins in regulating pressure sensing.