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Mechanical Force Induced Calcium Signals are Extracellular Matrix (ECM) Specific in Arteriolar Vascular Smooth Muscle Cells (VSMC)
Author(s) -
Sun Zhe,
Li Zhaohui,
Meininger Gerald A.
Publication year - 2010
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.24.1_supplement.779.3
Subject(s) - extracellular matrix , fibronectin , chemistry , vascular smooth muscle , integrin , biophysics , mechanotransduction , adhesion , type i collagen , matrix (chemical analysis) , focal adhesion , microbiology and biotechnology , cell , smooth muscle , biochemistry , biology , endocrinology , organic chemistry , chromatography
It has been proposed that integrin‐mediated mechanisms contribute importantly to mechano‐regulation of Ca 2+ in VSMC. An integrated atomic force (AFM)‐confocal microscopy system was used to investigate the relationship between pulling force applied through integrin‐ECM protein adhesions and Ca 2+ signals in VSMCs isolated from rat cremaster feed arterioles. AFM attached microbeads (5 μm) were coated with fibronectin (FN), collagen type I (CNI) or type IV (CNIV) and positioned on a VSMC for 20 min resulting in formation of a focal adhesion between the cell and microbead. Application of step increases in pulling force (800~3000 pN) to FN or CNI adhesions induced Ca 2+ transients that increased in frequency and intensity with applied force. An increase in the total area of the cell showing transients was only observed with FN at high force (3000 pN). No changes in Ca 2+ behavior were observed during pulling through CNIV or albumin coated beads. However, detachment of the CNIV bead from cell surface increased the area of Ca 2+ transient activity, which was not observed with FN‐ and CNI‐coated beads. These data suggests that mechanotransmission through FN‐, CNI‐ and CNIV‐ alters Ca 2+ signaling in VSMC in a force and ECM dependent manner. (Supp. by NIH HL58690 to G.A. Meininger and AHA 0765481Z & 0835676N to Z. Sun)