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Biocompatibility of crosslinked aerogels of variable densities with blood platelets and vascular endothelial cells
Author(s) -
Morton Blake Edward,
Jarrett Ellen,
Lu Hongbing,
Yin Wei,
Rubenstein David Alan
Publication year - 2010
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.24.1_supplement.779.1
Subject(s) - aerogel , biocompatibility , platelet , platelet activation , chemistry , flow cytometry , biophysics , materials science , thrombin , porosity , biomedical engineering , nanotechnology , immunology , composite material , medicine , organic chemistry , biology
Evaluating the vascular biocompatibility of crosslinked aerogels (X‐aerogels) can result in the development and improvement of blood implantable devices. X‐aerogels of variable densities can be used to optimize the overall compatibility of the material with respect to material processing. Initial research has shown that X‐aerogels do not alter platelet activation or aggregation. Here, platelets were incubated with X‐aerogel samples of different densities for up to 24 hours. Optical light aggregometry was used to quantify the rate and total extent of platelet aggregation at 0, 0.5, 1, 2, 4, 6, and 24 hours. Platelet activation state (PAS) was quantified by the modified prothrombinase assay under static and flow conditions, which measures thrombin generation. Flow cytometry was performed to quantify the surface protein expressions of CD41 and CD62P of platelets to identify a possible mechanism for altered cellular responses. A cell cytotoxicity assay and MTT assay was used to determine the endothelial cell compatibility with X‐aerogel samples. Aggregometry and PAS results suggest that X‐aerogels of variable densities do not alter platelet functions. Endothelial cells were also compatible with X‐aerogel samples. Based on these results, we conclude that X‐aerogel is a viable biomaterial and future research will focus on determining the optimal density and porosity of X‐aerogel to maximize biocompatibility.