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Bone marrow stem cell transplant to improve cardiac function in SS/MCWi rats with hypertensive heart disease
Author(s) -
Parker Sarah Josepha,
Greene Andrew S
Publication year - 2010
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.24.1_supplement.778.1
Subject(s) - medicine , bone marrow , heart failure , cardiac function curve , myocardial infarction , hypertensive heart disease , cardiology , bone marrow stem cell , fibrosis , stem cell , urology , biology , genetics
Bone marrow derived stem cell (BMSC) injection has been shown to reverse cardiac damage and prevent or slow the progression of heart failure after myocardial infarction. It is not known if BMSC transplant can improve function in non‐ischemic heart failure such as that induced by hypertension. The purpose of this study was to examine if BMSC transplant could improve left ventricular structure and function in SS/MCWi rats with hypertensive heart disease. At 8 weeks of age, SS/MCWi rats were placed on 8% NaCl diet. After 4 weeks on 8% NaCl, rats received either 3 x 10 7 bone marrow mononuclear cells (BMSC, N=5) from a non‐hypertensive donor rat or vehicle (VEH; N=4) via tail vein and followed with weekly cardiac ultrasound exams and terminal histology. BMSC improved survival over the 8 week study (Mean survival time 44 +/− 4 days BMSC vs 35 +/− 2.5 days VEH). While baseline and 4‐week values were equal between groups, BMSC treated rats had significantly higher % fractional shortening than vehicle treated rats 1‐week after injections (57.1 +/− 2.3% BMSC versus 49.2 +/− 1.0 VEH). Fibrosis was significantly reduced in rats receiving BMSC injection (10.7 +/− 1.7 % in BMSC versus 15.6 +/− 1.7 % for VEH), while the number of capillaries‐per‐fiber was increased (4.2 +/− 0.27 BMSC versus 3.9 +/− 0.08 VEH). These data suggest that the transplant of BMSC can improve cardiac function during hypertensive heart disease in the SS/MCWi rat on 8% NaCl diet.