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Vasoprotective effects of caloric restriction in aging
Author(s) -
Csiszar Anna,
Pinto John,
Ballabh Praveen,
Pearson Kevin,
Cabo Rafael,
Ungvari Zoltan
Publication year - 2010
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.24.1_supplement.775.7
Subject(s) - vasoprotective , oxidative stress , endothelial dysfunction , inflammation , endocrinology , medicine , chemistry , tumor necrosis factor alpha , nitric oxide
Endothelial‐dysfunction, oxidative stress and inflammation are associated with vascular aging and promote the development of cardiovascular disease. To determine whether caloric restriction (CR) exerts vasoprotective effects in aging, aortas of ad libitum (AL) fed young and aged and CR‐aged F344 rats were compared. Aging in AL‐rats was associated with impaired acetylcholine‐induced relaxation, vascular oxidative stress and increased NF‐êB‐activity. Lifelong CR significantly improved endothelial function, attenuated vascular ROS production, inhibited NF‐kB activity and down‐regulated inflammatory genes. In coronary‐arterial endothelial cells (CAECs) cultured in the presence of AL‐serum TNFá elicited oxidative‐stress, NF‐kB‐activation and inflammatory gene expression. By contrast, treatment of CAECs with CR‐serum attenuated TNFá‐induced ROS generation and prevented NF‐kB‐activation and induction of inflammatory genes. siRNA‐knockdown of SIRT1 mitigated the anti‐oxidant and anti‐inflammatory effects of CR‐serum. CR exerts anti‐oxidant and anti‐inflammatory vascular effects, which are likely mediated by circulating factors, in part, via a SIRT1‐dependent pathway.