Premium
Mouse models of variability in vascular remodeling: collateral networks in spinotrapezius muscle ischemia
Author(s) -
Mac Gabhann Feilim,
Peirce Shayn M.
Publication year - 2010
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.24.1_supplement.774.25
Subject(s) - arteriole , ischemia , arteriogenesis , ligation , anatomy , population , microcirculation , medicine , biology , pathology , environmental health
We used inbred and outbred mouse strains as models for interindividual variability in microvascular network structure. Other groups previously identified remodeling responses of mouse strains to ischemic ligation of hindlimb arteries. Our studies induced arterial ischemia in the mouse spinotrapezius, a model system that allows for the whole‐mount, en‐face visualization of the entire muscle microvasculature. We observed two groups of mice: ischemia‐vulnerable, characterized by dendritic arteriolar structure, and ischemia‐protected, characterized by frequent arteriole‐to‐arteriole connections. During vascular remodeling, mice that were characterized as “ischemia‐vulnerable” re‐routed blood to the ischemic tissue via the capillaries, a subset of which increased in diameter and became arterialized. Mice in the “ischemia‐protected” population re‐routed blood flow via arterioles, which showed tortuosity and diameter increases, while capillaries were unchanged. Each outbred CD1 mouse fell into one of the above two categories, siblings having different vascular phenotypes. We developed a computational model to quantify the protective effect of arteriole‐to‐arteriole connections, and found that only a small number of these higher‐diameter bridges are needed to provide protection to the tissue. Support provided by: NIH‐HL082838‐02 , NIH‐T32‐ HL007284 and NIH‐K99‐HL093219.