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Vitamin D regulates cathelicidin (CAMP) but not interleukins (IL12A or 27) mRNA, levels in HTR‐8/SVneo human placental trophoblasts in vitro
Author(s) -
Caty Jane T,
Jones Sara,
Bran Patsy M.
Publication year - 2010
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.24.1_supplement.774.17
Subject(s) - cathelicidin , trophoblast , biology , placenta , medicine , endocrinology , vitamin d and neurology , immune system , messenger rna , innate immune system , immunology , fetus , biochemistry , gene , pregnancy , genetics
The placenta synthesizes active 1,25‐dihydroxyvitamin D [1,25(OH) 2 D], which may suppress the maternal immune system by regulating placental immunomodulatory peptides and placental hormonal function. Whether it also regulates placental growth is unknown. To determine its regulation of placental immunomodulatory peptides and cell growth, HTR‐8/SVneo human trophoblast cells were cultured with 100–1000 nmol/L of 1,25(OH)D 3 or 25(OH)D 3 or 0.1% ethanol (C) for 24 h. VEGF, Ki‐67 (marker of cell proliferation), interleukins (IL‐12A and IL‐27) and cathelicidin antimicrobial peptide (CAMP) mRNA levels were measured by RT‐PCR. Cellular protein and DNA were measured using Lowry and spectrofluorometric assays. 1,25(OH) 2 D 3 and 25(OH)D 3 increased CAMP mRNA levels 1 fold (p<0.001 by ANOVA), but did not affect IL‐27 and IL‐12 A mRNA levels. 1,25(OH) 2 D 3 (100‐1000 nmol/L) and 25(OH)D 3 (500–1000 nmol/L) were equally effective in regulating CAMP mRNA, suggesting that HTR‐8/SVneo human trophoblasts activate 25(OH)D 3 . Neither 1,25(OH) 2 D 3 nor 25(OH)D 3 altered cellular DNA, protein‐to‐DNA ratio or Ki67mRNA levels, suggesting that it does not alter growth in 24 h. Neither altered hCG mRNA levels in 24 h. Vitamin D regulates placental CAMP expression and may, thus, modulate maternal innate immunity, which might be important in protecting maternal and placental tissue from microbial infection.