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Molecular Expression and Distribution of Calcium‐Activated Chloride Channel TMEM16A in Mouse Heart
Author(s) -
Zhang Mi,
Yoon Carol,
Kuhn Andrew R.,
Dai Yanping,
Ye Linda,
Singer Cherie A.,
Burkin Dean J.,
Duan Dayue Darrel
Publication year - 2010
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.24.1_supplement.770.7
Subject(s) - ventricle , messenger rna , western blot , atrium (architecture) , gene expression , microbiology and biotechnology , biology , chemistry , gene , pathology , medicine , biochemistry , atrial fibrillation
Background Calcium‐activated Cl − channels (CaCCs) are important in many cellular and physiological functions and in certain cardiac diseases. But the molecular identity of CaCCs in the heart is still elusive. Recent evidence suggests TMEM16A as the gene encoding the pore subunit of CaCCs. In this study, the molecular expression and tissue distribution of TMEM16A in mouse heart were quantitatively analyzed. Methods Total RNA was isolated from atria and ventricles of mouse heart (C57/BL6) and subjected to a quantitative real‐time PCR and Western blot analysis. TMEM16A mRNA and protein expression was calculated relative to 18S rRNA and GAPDH expression, respectively, within the same tissues. Results The transcriptional level of TMEM16A as estimated by mRNA ratio of TMEM16A/18s rRNA was highest in the left atrium (2.13±1.5, n=3), followed by right atrium (1.31±0.38), right ventricle (0.11±0.01), and left ventricle (0.051±0.004). Immunoblots of atrial and ventricular wall protein extracts demonstrated TMEM16A immunoreactivity at 113 kDa. The protein expression levels of TMEM16A were consistent with the same tissue‐distribution pattern as the transcriptional levels. Conclusion These results suggest that TMEM16A is heterogeneously expressed in mouse cardiac tissues, which may be responsible for the different functional activity of CaCCs in different tissues of the heart. (Supported by NCRR P‐20 RR‐15581)