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Cytoprotection of human endothelial cells from oxidative stress by polyphenols: the role of gene expression versus direct antioxidant effect
Author(s) -
Wang Xinyu,
Bynum James,
Stavchansky Salomon,
Dubick Michael,
Hackman Robert,
Keen Carl,
Bowman Phillip
Publication year - 2010
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.24.1_supplement.760.3
Subject(s) - resveratrol , caffeic acid phenethyl ester , oxidative stress , curcumin , cytoprotection , caffeic acid , antioxidant , chemistry , polyphenol , biochemistry , menadione , hydroxytyrosol , pharmacology , biology
Polyphenols have been implicated in protecting cells against oxidative stress. Several polyphenols including caffeic acid phenethyl ester (CAPE), curcumin, resveratrol, caffeic acid, catechin, and Oligonol ™ , a commercial source of a mixture of polyphenolics were investigated for their cytoprotective effects in an in vitro model of menadione‐induced oxidative stress in human umbilical vein endothelial cells and for the transcriptional activity. CAPE, curcumin, and resveratrol showed good dose‐dependent cytoprotection against menadione‐induced cytotoxicity, whereas oligonol, (+)‐catechin, and caffeic acid did not. The results of ‘direct’ antioxidant capacities of those compounds by 2, 7‐dichlorofluorescin assay indicated that most compounds tested showed good free radical scavenging abilities except resveratrol. However, ‘direct’ antioxidant activity did not correlate well with their cytoprotective effects. Gene expression analysis with whole genome microarrays and submission of statistically significant results to Ingenuity Pathway Analysis showed that a number of genes were up‐ or down‐regulated by these compounds in unique and common patterns, which may account for their beneficial effects, in particular the heat shock protein family and IL‐8 signaling pathway.