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Modulatory effects of lipoic acid and selenium against cadmium‐induced biochemical alteration in testicular steroidogenesis
Author(s) -
Nassar Noha N,
ELMaraghy Shohda A
Publication year - 2010
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.24.1_supplement.759.1
Subject(s) - selenium , lipoic acid , endocrinology , medicine , antioxidant , testosterone (patch) , chemistry , cadmium , hormone , luteinizing hormone , cadmium chloride , biochemistry , organic chemistry
Exposure to toxic metals, especially Cadmium (Cd), a major environmental pollutant, has become an increasingly recognized source of illness worldwideSince antioxidant therapy provides potential protective role against metal induced testicular damage, hence the current study aimed at investigating the protective effects of lipoic acid and selenium in Cd‐induced testicular damage. Accordingly, male Wistar rats were allocated into four groups (n=8; each). One group received saline and served as control, while the other three groups were given CdCl 2 (2mg/kg, i.p. for 28 days) either alone or in combination with (i) lipoic acid (35mg/kg, p.o) or (ii) selenium (as sodium selenite, 0.35mg/kg, p.o) from the first day of Cd administration till the end of the experiment. Serum testosterone, luteinizing hormone and follicular stimulating hormone levels were significantly decreased in the Cd‐exposed rats as well as the activity of testicular key androgenic enzymes such as (Δ 5 3β‐HSD) and 17 β‐HSD as compared the control counterparts. In addition, the activities of testicular marker enzymes were significantly altered in cadmium treated animals. Significant reduction in body weight, testis weight, and testicular antioxidant status was also observed in Cd‐exposed rats. Lipoic acid and selenium significantly restored serum testosterone level and testicular activity of Δ 5 3β‐ HSD, 17 β‐HSD Moreover, we demonstrate alterations in some testicular metal concentrations that were corrected by either selenium or lipoic acid. The biochemical parameters were further confirmed with histopathologial findings. In conclusion, the present study demonstrated the beneficial influences of lipoic acid and selenium in reducing harmful effects of Cd in rats’ testes.