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Detection of differential splicing of Abcg8 in liver of lactating rats compared to virgin controls using exon arrays
Author(s) -
Athippozhy Antony Thomas,
Zhao Tianyong,
Coy Donna J,
WootonKee Clavia Ruth,
Jungsuwadee Paiboon,
Stromberg Arnold J,
Vore Mary
Publication year - 2010
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.24.1_supplement.758.3
Subject(s) - exon , splice , rna splicing , alternative splicing , biology , cholesterol , fatty liver , gene , microbiology and biotechnology , endocrinology , medicine , genetics , rna , disease
Lactating dams have an increased demand for cholesterol for secretion into milk, bile acid synthesis and incorporation into cellular membranes. We used Affymetrix Rat Exon 1.0ST® analysis to investigate mRNA expression in liver of lactating (L) vs female control (C) rats. Analysis of splicing variance (ANOSVA) revealed 59 differentially alternatively spliced genes (p<0.001). Abcg5 and Abcg8 function as a heterodimer to export cholesterol into bile, creating a major pathway for cholesterol clearance. Exon array analysis indicated reduced mRNA of Abcg5 and Abcg8 in L vs C, and demonstrated differential alternative splicing of Abcg8 (ANOSVA p=0.0002), but not Abcg5. Screening of calculated expression levels for each exon indicated that exons 6 and 10 were alternatively spliced in L; RT‐PCR confirmed differential splicing. One splice variant detected in L, but not C, showed deletion of part of exons 4 and 9 and all intervening exons by sequencing the RT‐PCR products from the splice variant. Loss of Abcg5/g8 would provide a mechanism for controlling cholesterol loss in bile during lactation. (DK46923)