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Roll of Toll‐like Receptor 9 in Mouse Lung Inflammation in Response to Chicken Barn Air
Author(s) -
Schneberger David,
Aulakh Gurpreet,
Channabasappa Shankaramurthy,
Caldwell Sarah,
Singh Baljit
Publication year - 2010
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.24.1_supplement.753.7
Subject(s) - tlr9 , inflammation , barn , lung , tlr4 , immunology , lipopolysaccharide , biology , barn owl , tumor necrosis factor alpha , andrology , respiratory system , pathology , microbiology and biotechnology , medicine , gene expression , gene , anatomy , paleontology , biochemistry , civil engineering , dna methylation , predation , engineering
Exposure to barn air is an occupational hazard that results in lung dysfunction in barn workers. These respiratory symptoms are typically associated with endotoxin, but within these environments gram negative bacteria may constitute only a small portion of the species and quantity of microorganisms. In contrast, un‐methylated DNA by can be found in all bacteria, most viruses and, and mould. Therefore, we investigated the role of TLR9, which binds to un‐methylated DNA, in barn‐air induced lung inflammation. First we used immunohistology, immuno‐electron microscopy and in situ hybridization to show expression of TLR9 on bronchial epithelium, alveolar septal cells, and alveolar macrophage of mouse and human lungs. Using a TLR9‐deficient mouse model, these animals were exposed to barn air for 8 hours/day for 1, 5, or 20 days. Examination of bronchiolar lavage and serum against a panel of six common cytokines (IL‐1β, IL‐6, IL‐10, IL‐12, TNF‐α, and IFN‐γ) showed no significant differences after a single day exposure. While TNF‐α (p=0.06) levels in TLR9‐deficient mice were reduced in blood and lavage fluids after 5 and somewhat reduced at 20 days of exposure (p=0.14), IFN‐γ at 5 days(p=0.06) remained reduced after 20 days (p=0.05). Taken together, our data shows expression of TLR9 in mouse and human lungs and that TLR9 may partially contributes to inflammation induced following exposure to chicken barn air. Grant Funding Source : NSERC Discovery Grant