Premium
The Effects of Cell Lysates of Bifidobacterium longum (BL730) and Lactobacillus casei (E5) on in vitro Stimulation of Mouse Immune Cells
Author(s) -
Lee WooSik,
Hwang SeongGu,
Abanto Oliver Diaz,
Park Seung Yong
Publication year - 2010
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.24.1_supplement.753.1
Subject(s) - bifidobacterium longum , lactobacillus casei , immune system , cytokine , in vitro , cell culture , chemistry , bifidobacterium , nitric oxide , microbiology and biotechnology , macrophage , biology , lactobacillus , immunology , biochemistry , genetics , organic chemistry , fermentation
The present study was conducted to investigate in vitro mechanisms involved in the immunostimulatory effects of Bifidobacterium longum and Lactobacillus casei . RAW 264.7 macrophage cell line, primary cultures of BALB/c peritoneal macrophages and neutrophils were treated with different concentrations of B. longum lysates ( BL ) or L. casei lysates ( LC ). Nitric oxide ( NO ) production and IL‐6 cytokine expression were measured using nitrite assay and sandwich ELISA, respectively. Neutriphils and macrophages were treated with or without BL or LC, and the phagocytotic activities were measured using FACS. The antioxidant activity of BL and LC were analysed using DPPH assay. Both lysates induced NO production in both cell line and primary cultured cells. The NO production in primary cultures of macrophages and neutrophils were significantly higher (p<0.05) when treated with 500 μg/mL of LC than those treated with the same dose of BL. The BL stimulated IL‐6 cytokine expression significantly higher than LC in both cell line and primary cultured cells. The LC enhanced phagocytotic activity of neutrophils more strongly than BL. The free radical scavenging activity of LC was significantly higher (p<0.05) than that of BL. The results of the present study suggest that both B. longum and L. casei can modulate immune response in mouse, but with different mode of actions.