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Efficacy of Ozone Therapy Against the Formation of Peritoneal Adhesion in Rat
Author(s) -
Uysal Bulent,
Demirbag Suzi,
Cayci Tuncer,
Poyrazoglu Yavuz,
Topal Turgut,
Guven Ahmet,
Korkmaz Ahmet
Publication year - 2010
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.24.1_supplement.751.5
Subject(s) - ozone therapy , malondialdehyde , medicine , oxidative stress , adhesion , superoxide dismutase , surgery , peritoneal cavity , andrology , gastroenterology , pathology , chemistry , alternative medicine , organic chemistry
Peritoneal adhesion (PA) is a consequence of wound healing that sometimes causes severe postoperative complications. Despite all the preventive measures and agents, PA has still not been eliminated completely. The aim of this study was to evaluate the effect of the medical ozone therapy (MOT) on the development of postoperative peritoneal and uterine tube adhesions. MATERIAL AND METHOD Twenty one female Wistar‐Albino type rats were used in this study. The rats were randomly divided into three groups of sham‐operated, PA and PA+MOT. After performing a lower midline abdominal incision, a standard uterine lesion was created by electrocauterization in the PA and PA+MOT groups. The PA+MOT group received 0.7 mg/kg per day ozone/oxygen mixture intraperitoneally for a total of 3 days. At the 7th day, peritoneal washing fluids of rats were obtained for TNF‐alpha evaluation. Then all of the rats were killed, and uterine adhesions were staged according to the Leach scoring system. Uterine tissue samples were harvested for biochemical and histopathological evaluation. RESULTS In the PA+MOT group, the total adhesion score, oxidative stress markers including malondialdehyde, superoxide dismutase and glutathione peroxides were significantly lower than the PA group (P < 0.05). In addition, TNF‐alpha levels of peritoneal washing fluid were found decreased in the PA+MOT group than the PA group. CONCLUSION Medical Ozone Therapy significantly reduces the prevalence of peritoneal and uterine tube adhesion formation in rats by possible decreasing oxidative stress.