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Pharmacological and toxicological evaluation of methanolic extracts from leaves, steam and roots of a Cnidoscolus plant
Author(s) -
Arjona Carlos A.,
RodríguezFragoso Lourdes,
RosadoVallado Miguel,
FitzAranda Claudia,
MendozaRivera Brissa,
ReyesEsparza Jorge
Publication year - 2010
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.24.1_supplement.751.10
Subject(s) - candida albicans , viability assay , embryo , antifungal , yeast , chemistry , cell growth , biology , in vitro , botany , microbiology and biotechnology , biochemistry
The aim of this work was to evaluate antifungal activities, and the effect on viability, cell proliferation and embryotoxicity of a Cnidoscolus plant antifungal activities were evaluated through microdilution with broth method in different yeast strain. Effect on cell viability and the effect on cell proliferation were evaluated on two cell lines (renal and hepatic cells) at 24 h. Embryotoxicity was evaluated on chicken embryos (Gallus gallus). Leaves and roots extracts had antifungal activity against Candida albicans (752 y 10231 ATCC) with concentrations of 0.1 to 10000μg/mL. Both extracts were cytotoxic and decrease cellular proliferation at fourth day with concentrations higher than 50μg/mL. These methanolic extracts did not affect embryo viability with concentrations lowers to 100μg/mL However, they affected embryo development when the extracts were 100μg/mL or higher. It was observed a minor development, affectation of CNS, reduction of somites, absence or hypoplasia of optical and optical placodes. These results shown that the leaves and roots methanol extracts might have different components which are responsible for the antifungal activity and/or toxic activity.