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In vitro preliminary findings on the effect of biodegradable composite material in fibroblasts‐stimulated angiogenesis
Author(s) -
Pirraco Ana,
Rocha Ana Sofia,
Vieira André,
Vieira Joana,
Guedes Rui,
Soares Raquel
Publication year - 2010
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.24.1_supplement.750.4
Subject(s) - angiogenesis , fibroblast , biocompatibility , viability assay , ligament , biomedical engineering , wound healing , chemistry , in vitro , periodontal fiber , regeneration (biology) , microbiology and biotechnology , materials science , biophysics , anatomy , immunology , medicine , biology , biochemistry , dentistry , organic chemistry
The current ligaments treatment injuries has several drawbacks that prompted attempts to use synthetic materials in ligament replacements. Although fibroblasts do not comprise a large volume of ligament tissue, they have an important role in ligament tissue regeneration as they are able to model ECM and angiogenesis, therefore affecting the healing process. The aim is to appraise the biocompatibility of different biodegrable ligament composites (PGA‐PCL, PLA‐PCL, PDO, PGA) with the fibroblast‐mediated angiogenic process in vitro. HFF1 fibroblast cells were cultured on plates coated with each of the composite and cell viability was examined. Media from HFF1 cells incubated with composite material was removed and used to appraise the effect on angiogenesis (aortic ring assay and capillary‐like structures formation assay). No significant difference in cell viability was found for any composite tested. Aortic ring results suggested no disturbance of the angiogenic process. These findings were corroborated by the formation of cord‐like structures assay, as no significant difference in the number of structures was found in comparison to controls. Overall, our preliminary findings indicate that the composites do not promote nor prevent angiogenesis, being thus a good tool for the development of ligament composites. Supported by FCT (PTDC/EME‐PME/70155/2006)