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The role of mechanical forces in stem cell differentiation to vascular lineage
Author(s) -
Nikmanesh Maria,
Shi ZhongDong,
Tarbell John M
Publication year - 2010
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.24.1_supplement.750.13
Subject(s) - microbiology and biotechnology , endothelial stem cell , matrigel , cell sorting , stem cell , embryonic stem cell , vasculogenesis , in vitro , chemistry , biology , biochemistry , progenitor cell , gene
The overall goal of our study is to evaluate the role of wall shear stress (WSS) and cyclic strain (CS) on the development of vascular cells from mouse embryonic stem cells (MESCs) differentiated in vitro . The aim is to generate an early marker of endothelial cells (ECs), platelet endothelial cell adhesion molecule‐1 (PECAM‐1 + cells), from ESCs differentiated in vitro , subjecting these cells to WSS and CS, and analyzing the functionality of these cells by testing their permeability to solutes of varying molecular weight and expression of key components of the inter‐endothelial tight junctions. If we are successful, the results will make an important contribution to vascular tissue regeneration. Results 1) We have been able to sort PECAM‐1 + cells from the rest of the MESC culture; 2) The endothelial‐like cells display border staining for VE‐cadherin; 3) ES‐derived endothelial cells form multidimensional tubular structures on Matrigel; and 4) ES‐derived endothelial cells uptake acetylated low density lipoprotein (ac‐LDL). Conclusion Our sorting for PECAM‐1 + cells has been successful and these cells express hallmark of the endothelial phenotypes. We can use these cells for evaluating the role of WSS and CS on their development into mature endothelial cells. Supported by: NIH/NHLBI 1 RO1 HL086543‐01 and State of New York Department of Health (NYSTEM)