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Using routine blood chemistry results to estimate changes in plasma AA during experimental endotoxemia
Author(s) -
Price Kathryn L.,
Utt Matthew D.,
Lee Hanbae,
Escobar Jeffery
Publication year - 2010
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.24.1_supplement.740.22
Subject(s) - chemistry , creatinine , blood urea nitrogen , blood sampling , medicine , blood plasma , plasma concentration , amino acid , zoology , endocrinology , biochemistry , biology
Plasma amino acids (AA) markedly change during infection, but they are rarely monitored during hospitalization. Arg, Glu, Ala, and the aromatic AA have important immunological functions. Young pigs (26.9±1.1 kg, n=9) were fitted with an indwelling jugular catheter for blood sampling. After a 2‐h feeding period, pigs were given 10 μg/kg BW of E. coli ‐derived LPS i.v. Plasma AA and several blood metabolites (e.g., creatinine, urea N, protein, P, Ca, TG, HDL, glucose, lactate, etc.) were measured every 2 h for 12 h. Quadratic changes ( P <0.04) in Pro, Arg, Tyr, Val, Leu, Asn, Ile, Met, Phe, Orn, and Trp were determined after LPS injection. Plasma total protein (PTP, r 2 =0.003 to 0.18) and urea N (BUN, r 2 =0.001 to 0.23) were poorly correlated to plasma AA levels during an LPS challenge. The use of multiple blood chemistry results better ( P <0.01) accounted for plasma levels of Arg (R 2 =0.61), Ala (R 2 =0.80), Trp (R 2 =0.75), Phe (R 2 =0.77), Tyr (R 2 =0.67), Met (R 2 =0.62), Lys (R 2 =0.30), Leu (R 2 =0.71), and Val (R 2 =0.69) than PTP or BUN alone. These findings suggest that plasma AA could be estimated using various routine blood chemistry results. Funding: Virginia Agricultural Experiment Station. Grant Funding Source : Virginia Agricultural Experiment Station