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Plasma sulfur amino acids and glutathione response to methionine loading in HIV‐infected patients
Author(s) -
BorgesSantos Maria Dorotéia,
Pereira Paulo Camara Marques,
Burini Roberto Carlos
Publication year - 2010
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.24.1_supplement.740.10
Subject(s) - glutathione , methionine , chemistry , medicine , plasma concentration , endocrinology , amino acid , blood plasma , thiol , biochemistry , enzyme
BACKGROUND HIV patients present lower plasma levels of GSH and all sulfur amino acids (SAA). We hypothesize that methionine (Met) supply would restore mostly of the GSH biosynthesis. PURPOSE To examine the responses of plasma GSH and SAA to an acute load of Met (ML). METHODS In 20 control subjects and 12 HIV‐infected patients under HAART, plasma SAA and GSH were measured in response to a ML (0.1 g/kg). Blood samples were taken before, 2h and 4 h after ML and the plasma Met disappearance rate was measured by area under the curve (AUC). The groups were compared using Wilcoxon's test (p<0.05). RESULTS The baseline GSH and SAA levels were significantly lower in HIV than controls. These differences persisted even after the ML (Met: 1811 vs 1555mm 2 , Hcy: 99 vs 118mm 2 ; Cys: 1179 vs 1537mm 2 ; Tau: 385 vs 443mm 2 ). The response profile to ML was similar in both groups with an increasing in postload Tau and no changes in plasma GSH or its redox status. CONCLUSIONS In the presence of ML Cys is not fully converted to GSH being probably turned aside to Tau. Supported by Fapesp, CNPq.