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Reproducibility in growth of breast and prostate cells stimulated with serum taken at different points in time from individuals on their habitual diets
Author(s) -
Esfahani Amin,
Srichaikul Korbua,
Bashyam Balachandran,
Kendall Cyril WC,
Archer Michael C,
Jenkins David JA
Publication year - 2010
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.24.1_supplement.728.6
Subject(s) - lncap , cell growth , prostate cancer , medicine , endocrinology , reproducibility , physiology , cell , volunteer , cancer , breast cancer , stimulation , body mass index , bioassay , oncology , prostate , biology , chemistry , biochemistry , genetics , chromatography , agronomy
Background While dietary components have been linked to cancer, the total impact of specific diets on cancer remains controversial. Therefore, we undertook a series of studies to develop a reliable ex vivo bioassay for assessing the effect of diet on cancer cell growth. Various types of human cancer cell lines were treated with serum from subjects on their habitual diets. Methods Eighteen subjects (10 males; 8 females) provided us with three fasting blood samples, each separated by one week. Subjects were asked to maintain their habitual diet and exercise regimen for the length of the study. Three cell lines (MCF‐7, MCF‐10A, LNCaP) were then incubated in media containing the serum samples (10%). After 72 hours, cell growth was measured using the MTS colorimetric assay. Results The stimulation of cell growth was relatively constant for a given individual over the 3‐week period. There were, however, statistically significant inter‐individual differences in serum‐stimulated cell growth in all three cell lines. Subject age and body mass index (BMI) were not correlated with cell growth. Conclusion These findings suggest that the consistency of results for a given individual will allow differences in breast and prostate cell growth resulting from specific diets to be detected using this method. (Funding provided by Canada Research Chair discretionary funds)