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Effect of Methyl Nutrients on Feline Lymphoma Growth
Author(s) -
Mabasa Lawrence,
Cho Kyongshin,
Fowler Andrea W.,
Park Chung S.
Publication year - 2010
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.24.1_supplement.725.9
Subject(s) - lymphoma , apoptosis , cell growth , biology , cell culture , cell , cancer research , caspase , medicine , microbiology and biotechnology , programmed cell death , immunology , biochemistry , genetics
Feline lymphoma is one of the most frequently diagnosed tumors in cats. Lipotropes are dietary methyl donors and cofactors that are involved in one‐carbon metabolism and DNA methylation. However, little is known about their role in feline lymphoma. We hypothesized that supplementation of lipotropes would modulate the DNA methylation status and the expression of genes involved in cell growth and apoptosis of feline lymphoma cells. The objective of the study was to determine if lipotropes affect growth of feline lymphoma cells: entailing examining a correlation between lymphoma cell growth and caspase‐3 enzyme activity. Feline lymphoma cell lines, F1B and FeLV‐3281, were cultured and treated with 20 times the level of each lipotrope contained in the basal culture medium (control). Cell growth and caspase‐3 activity were measured. Lipotropes significantly reduced cell growth (45.4%) and increased caspase‐3 activity (4.9%, P = 0.01) of F1B cells after 72 hours. However, cell growth and caspase‐3 activity of FeLV‐3281 were not affected by lipotropes. These results suggest that methyl nutrients may play a role in reducing cell growth of F1B, but not FeLV‐3281. Further studies are warranted to determine the mechanism underlying the inhibitory effect of lipotropes on F1B lymphoma cell growth. Supported by Morris Animal Foundation (D08FE‐047).