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Whole blood gene differences between osteoarthritic dogs compared to healthy geriatric dogs
Author(s) -
Frantz Nolan,
Hahn Kevin,
Macleay Jen,
AlMurrani Sam
Publication year - 2010
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.24.1_supplement.725.5
Subject(s) - osteoarthritis , lameness , medicine , articular cartilage , gene , cartilage , arthritis , pathology , biology , surgery , anatomy , genetics , alternative medicine
A study was conducted to determine differences in 29 healthy geriatric dogs (initially 13.51 ± 1.66 kg, 10.7 ± 2.33 years) with no radiographic evidence of arthritis or lameness history compared with 31 arthritic dogs (initially 13.5 ± 1.27 kg, 11.0 ± 2.23 years) with varying degrees of radiographic evidence of osteoarthritis and a history of lameness. All dogs were fed a maintenance control food for 28 days prior to sample collection. Blood samples were drawn into PAXgene blood RNA tubes at day 28 and RNA was extracted according to the procedures provided in the PAXgene RNA Kit Handbook (Qiagen, Valencia, CA). Probes were generated based on the canine genome sequencing for 89 arthritis related genes for RT‐PCR analysis based on previous literature. Transcripts having a P < 0.05 (following a false discovery rate adjustment) and a fold change range of at least 1.25 were considered different among the two groups. Analysis of the RNA found differences in 23 genes between the blood of osteoarthritic and healthy geriatric dogs. Of the genes identified, 22 were down‐regulated and 1 up‐regulated. Several of these genes have also been analyzed for in canine articular cartilage, however, the majority of them are elevated in OA vs healthy cartilage. Similar to human data, an inverse relationship of genes associated with osteoarthritis between blood and articular cartilage may exist in the dog.