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Atherogenic Diet Promotes Liver Inflammation, Fatty Liver Formation & Atherogenesis in LDL Receptor Null Mice
Author(s) -
Wang Shu,
Wu Yi Chieh,
Wu Dayong,
Matthan Nirupa R.,
LamonFava Stefania,
Srinivasan Iswarya,
Reed Debra,
Lichtenstein Alice
Publication year - 2010
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.24.1_supplement.724.8
Subject(s) - medicine , endocrinology , cholesterol , triglyceride , ldl receptor , chemistry , inflammation , cytokine , fatty liver , fatty streak , receptor , biology , lipoprotein , disease
The effect of an atherogenic diet on hepatic cytokine production in relation to aortic lesion formation was assessed in LDL receptor null (LDLr−/−) mice. Mice (n=11/group) were fed an atherogenic (20% fat, 0.2% cholesterol, w/w) or control (4% fat, 0.02% cholesterol, w/w) diet for 32 weeks. Cytokine concentrations and tissue lipid content were measured by ELISA and enzymatic methods, respectively. The atherogenic diet, relative to the control diet, significantly increased the hepatic production of interleukin‐6 (IL‐6, 1.4‐fold, P<0.05) and tumor necrosis factor alpha (TNFα, 2.0‐fold, P<0.05). The atherogenic diet, relative to the control diet, also resulted in significantly higher content of liver triglyceride (3.5‐fold, P<0.01), total cholesterol (4.2‐fold, P<0.01) and esterified cholesterol (7.9‐fold, P<0.001). Greater accumulation of aortic esterified cholesterol (6.4‐fold, P<0.001) and higher plasma concentrations of TNFα (4.4‐fold, P<0.01) and IL‐6 (2.9‐fold, P<0.001) were found in the mice fed the atherogenic relative to control diet. These data suggest that the atherogenic diet‐induced liver inflammation partially contributed to higher plasma cytokine concentrations, which in turn were associated with atherogenesis in LDLr−/− mice.