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Expression of FoxP3 in Tregs cells from obese clinically healthy subjects
Author(s) -
Gomez Francisco Enrique,
ValenciaBarragan Karla Ivet,
Saldana Reyna,
FuruzawaCarballeda Janette,
KauferHorwitz Martha
Publication year - 2010
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.24.1_supplement.724.4
Subject(s) - foxp3 , adiponectin , leptin , medicine , inflammation , immune system , endocrinology , tumor necrosis factor alpha , flow cytometry , interleukin , immunology , obesity , cytokine , insulin resistance
Obesity is characterized by a low‐grade systemic inflammation due to increased levels of inflammatory proteins. FOXP3 code for a nuclear transcription factor in regulatory T cells (Tregs), which provide the “off” signal to maintain immune homeostasis and control inflammation. The aim of this study was to determine the levels of FoxP3 (mRNA and protein) in Tregs from obese clinically healthy patients to compare them with non‐obese controls. Methods We studied 18 (10♀,8♂) obese (O) patients (BMI 40±2, 31±9y) who had no other disease (diabetes, hypertension) or taking antinflammatory drugs. Control (C) group consisted of 46 (39♀,7♂) healthy non‐obese (BMI 21±1, 25±5y) volunteers. Serum levels of leptin, adiponectin, TNFa, IL‐1, IL‐6 and IL‐8 were determined by standard methods. Levels of FoxP3 mRNA by RT‐PCR, and CD4+FoxP3+ Tregs by flow cytometry. Results An inflammatory milleau (high levels of leptin, TNFa and IL‐6, and low of adiponectin) was present in the serum of O subjects. IL‐1 and IL‐8 were not different ( table). Despite a 25% increase in FoxP3 mRNA in O, %Tregs (CD4+FoxP3+) were very low compared to C. Results in the groups (×±sd) (* p ≤0.02)Control ObeseLeptin (pg/mL) 1129 ± 890 5293 ± 2482* Adiponectin (ng/mL) 4768 ± 1840 3367 ± 1196* TNFa (pg/mL) 5.5 ± 2.1 6.8 ± 2.0* IL‐1 (pg/mL) 0.9 ± 0.4 0.8 ± 0.5 IL‐6 (pg/mL) 4.7 ± 0.5 8.4 ± 4.5* IL‐8 (pg/mL) 3.3 ± 2.6 3.6 ± 0.9 FoxP3 mRNA 1.00 1.25 %Tregs (CD4+FoxP3+) 6.7 5.1Conclusion Increased transcription of FOXP3 (mRNA) does not result in normal values in Tregs in O subjects. This may partially explain their inflammatory status. (Partially supported by Conacyt‐Mexico).