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MODERATE EXERCISE AND ITS EFFECT ON THE SYSTEMIC IMMUNE RESPONSE ASSOCIATED TO HIGH FAT OR HIGH CARBOHYDRATE DIETS
Author(s) -
ValdésRamos Roxana,
MartínezCarrillo Beatriz E,
ArandaGonzález Irma I,
GuadarramaLópez Ana L,
Jarillo Rosa Adriana
Publication year - 2010
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.24.1_supplement.723.12
Subject(s) - medicine , immune system , endocrinology , cd8 , lymphocyte , immunology
High Carbohydrate (HCD) or high Fat (HFD) diets produce negative effects particularly on the immune system. Moderate exercise may be able to modulate these negative effects. Our objective was to evaluate the effect of moderate exercise on the peripheral immune system, associated to HCD or HFD intake. Three groups (n=8 each) of 21 day‐old Balb/c mice were fed control, HCD or HFD diets. During their fourth week of life, they were trained to swim with time increasing sessions (10, 15, 20, 25 and 30 minutes), after which they continued swimming for eight more weeks at 30‐minute sessions, five days a week with two days of rest. At 12 weeks of age, blood was obtained by intracardiac puncture for lymphocyte separation and analysis by flow cytometry (CD4+, CD8+ and CD19+). CD4+ lymphocytes were non‐significantly higher in HCD (49.68±3.01) and HFD (57.51±7.43), compared with controls (47.88±11.34). CD8+ were significantly higher (p<0.0001 and F=29.33) in the experimental diets (HCD =18.30±4.27; HFD 16.58±2.48) than controls (8.64±3.11). On the other hand, CD19+ were higher in the control group (18.46±6.23) than HCD (16.67±3.98) and HFD (13.16±3.89), p <0.001 and F =5.49. Moderate physical exercise associated with a HCD mainly increased T‐cytotoxic (CD8+) lymphocytes, whereas together with HFD it increased T‐helper (CD4+) cells. Both types of diets decreased B lymphocytes (CD19+). This project was financed by UAEM.