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Inhibitory effects of dietary luteolin on transendothelial migration of monocytes and formation of lipid‐laden macrophages
Author(s) -
Kim Dongshoo,
Kim Minsoo,
Kang SangWook,
Kang YoungHee
Publication year - 2010
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.24.1_supplement.721.2
Subject(s) - luteolin , foam cell , chemistry , macrophage , scavenger receptor , cell adhesion molecule , thp1 cell line , cell adhesion , adhesion , secretion , microbiology and biotechnology , cell , biochemistry , lipoprotein , in vitro , cell culture , biology , cholesterol , flavonoid , genetics , organic chemistry , antioxidant
In the development of atherosclerosis, the leukocyte transmigration begins with a multistep adhesion process that captures leukocytes from circulating blood. Oxidatively‐modified LDL (ox‐LDL) involved in development of atherosclerosis has been shown to facilitate leukocyte transmigration. Our previous study showed that flavone luteolin inhibited ox‐LDL‐triggered expression of cell adhesion molecules on vascular endothelial cells responsible for the leukocyte recruitment. This study investigated that luteolin disturbed scavenger receptor (SR) expression and retarded foam‐cell formation of THP‐1‐derived macrophages. In macrophages treated with 20 μg/mL ox‐LDL there was a heavy Oil red O staining, which was diminished by 10 μmol/L luteolin, repealing formation of lipid‐laden foam‐cells. Additionally, luteolin blunted uptake of 40 μg/mL fluorescent DiI‐labeled acetyl LDL in THP‐1‐derived macrophages. Such effects of luteolin entailed its blockade of macrophage SR induction. Furthermore, luteolin dampened TNF‐α‐elevated matrix‐degrading MMP‐9 secretion in THP‐1 cells, thus impeding their transendothelial migration. These results demonstrate that dietary luteolin was effective in curtailing SR‐mediated lipid‐laden macrophages and disturbing transendothelial migration of monocytes. Supported by the MKETD Project for Regional Strategic Planning.