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Anti‐cancer effect of Korean radish(Raphanus sativus L.) leaf extract through induction of apoptosis and down‐regulate Erb B2 signaling and Akt pathway in human breast MDA‐MB‐231 cell lines
Author(s) -
Chang MoonJeong,
Kim Boram,
Lee HyeSung,
Kim WooKyoung,
Cho Kangjin
Publication year - 2010
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.24.1_supplement.720.4
Subject(s) - apoptosis , raphanus , protein kinase b , signal transduction , cell growth , chemistry , cancer research , cell culture , biology , biochemistry , botany , genetics
The vegetative portion of radish have not been characterized in terms of its biological activity. In vitro anti‐cancer effect of aqueous extract from Korean radish leaf (EKRL) was evaluated in human breast MDA‐MB‐231 cell lines. EKRL preferentially inhibited the growth of cell lines in a dose‐dependent manner (p<0.05). In addition, EKRL treated MDA‐MB‐231 decreased expression of anti‐apoptotic Bcl‐2 protein and unchanged expression of pro‐apoptotic Bax protein, eventually leading a decrease in the Bcl‐2/Bax ratio. Phosphorylated Akt levels markedly reduced in cells treated with 100ug/L EKRL and total Akt levels decreased at 300ug/L. Furthermore, a dose‐dependent decrease in Erb B2 and Erb B3 levels was detected in EKRL treated cells. These results demonstrated that EKRL inhibits the proliferation of MDA‐MB231 through induction of apoptosis and down‐regulate Erb B2 signaling and the Akt pathway, making EKRL a potent candidate as new‐anti cancer food components. (This research was supported by Korea Rural Development Adminstration, project #2009AFT143783066)