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Effect of dietary iron deficiency and overload on hepatic ZIP transporter expression in rats
Author(s) -
Nam Hyeyoung,
Knutson Mitchell D
Publication year - 2010
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.24.1_supplement.717.9
Subject(s) - weanling , transporter , chemistry , iron deficiency , ferroportin , medicine , zinc , heme , biochemistry , dmt1 , endocrinology , biology , metabolism , gene , anemia , iron homeostasis , organic chemistry , enzyme
The mammalian ZIP (Zrt‐, Irt‐like Protein) family of transmembrane transporters consists of 14 members. Although ZIPs have been characterized largely by their ability to transport zinc, some ZIPs can transport other essential metal ions as well, including Fe 2+ and Mn 2+ . We sought to determine if iron deficiency or overload affected the expression of ZIP transporters in the liver, a main site of iron storage. Weanling male rats (n=6/group) were fed diets containing either 9 ppm Fe (deficient), 215 ppm Fe (control), or 2.8% carbonyl Fe (loaded). After 3 wk, liver non‐heme iron levels were 15, 87, and 5223 μg/g. Q‐RT‐PCR analysis of ZIP mRNA levels identified an 8‐fold induction in ZIP5 mRNA levels in iron‐loaded livers, and reciprocal regulation of ZIP10 by iron (twice as high in deficiency as in overload). The iron‐related changes in ZIP5 and ZIP10 expression were confirmed in a second study of rats fed AIN93G diets providing 10 ppm Fe, 50 ppm Fe, or 1.9 % carbonyl Fe, resulting in liver non‐heme Fe concentrations of 13, 30, and 1813 μg/g. These studies suggest that ZIP5 and ZIP10 may play a role in hepatic iron/metal homeostasis during iron deficiency and iron overload.