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Hepatic iron loading increases rat ZIP8 expression
Author(s) -
Wang ChiaYu,
Nam Hyeyoung,
Jenkitkasemwong Supak,
Duarte Stephanie
Publication year - 2010
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.24.1_supplement.717.18
Subject(s) - western blot , chemistry , cell , hepatocyte , zinc , transporter , ferrous , biochemistry , microbiology and biotechnology , medicine , biology , gene , organic chemistry , in vitro
Zip8 is a member of the ZIP (Zrt‐, Irt‐like Protein) family of metal‐ion transporters. Zip8 amino acid sequence shares approximately 60% homology to Zip14, which can transport both zinc and iron. Similar to Zip14, overexpression of rat Zip8 in HEK293T cells significantly enhances the uptake of iron. In the present study, Western blot analysis revealed ~3‐fold higher levels of Zip8 in iron‐loaded rat liver compared to iron‐normal controls (mean non‐heme iron concentrations of 1813 vs. 30 μg/g liver, respectively, n=6/group). Higher Zip8 protein levels were not associated with elevated Zip8 transcript abundance, suggesting post‐transcriptional regulation. In the H4IIE rat hepatocyte cell line, we further demonstrated that Zip8 is glycosylated and detectable at the cell surface. These data suggest that Zip8 may contribute to hepatic iron transport, particularly during states of iron overload. Supported by NIH. Grant Funding Source : NIH