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Effects of dietary conjugated linoleic acid (CLA) on intestinal absorption of vitamin A
Author(s) -
Giordano Elena,
Banni Sebastiano,
Quadro Loredana
Publication year - 2010
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.24.1_supplement.716.6
Subject(s) - chemistry , chylomicron , conjugated linoleic acid , vitamin , linoleic acid , olive oil , retinol , medicine , endocrinology , corn oil , food science , vitamin e , bioavailability , biochemistry , fatty acid , biology , lipoprotein , antioxidant , cholesterol , pharmacology , very low density lipoprotein
Conjugated linoleic acid (CLA) is a polynsaturated fatty acid of ruminant origin. We previously showed that CLA t10, c12 and CLA c9, t11 isomers elevate vitamin A levels in rodent liver. To investigate whether this hepatic vitamin A accumulation results from increased intestinal vitamin A absorption, we performed gavage experiments with wild‐type male mice. 12 hr prior the experiment, mice were injected with P‐407, a lipase inhibitor that blocks chylomicrons clearance. Next morning, they were given an oral bolus of all‐trans‐[3H] retinol (2× 106 cpm) and unlabelled all‐trans‐ retinol (6μg) in 100 μl of olive oil or olive oil + either CLA isomer (70:30, v:v). 3 hr after the gavage, mice were sacrificed to collect serum and tissues. HPLC analysis of retinoids and [3H]‐counts/min revealed reduced retinoids levels in the intestine of mice gavaged with olive oil + either CLA isomer compared to those given olive oil. In serum, retinoids levels were not different among groups, but triglycerides were elevated in the olive oil group compared to mice given olive oil + either CLA isomer. These results suggest that a single dose of dietary CLA could affect intestinal chylomicron assembly and/or secretion, as well as vitamin A incorporation in these particles. Further experiments are ongoing to elucidate the molecular mechanisms of this effect and the consequences of chronic dietary CLA intake on intestinal vitamin A absorption.