Premium
C1q/WOX1 signaling for superinduction of microvillus cluster formation
Author(s) -
He RueiYu,
Chang JeanYun,
Lin SingRu,
Lee MingHui,
Chen SheanJen,
Chang NanShan
Publication year - 2010
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.24.1_supplement.711.2
Subject(s) - microbiology and biotechnology , chemistry , cytosol , biology , biochemistry , enzyme
Complement C1q induces apoptosis of prostate DU145 and neuroblastoma cells overexpressing tumor suppressor WOX1 (known as WWOX or FOR), but not GFP control protein. By total internal reflection fluorescence microscopy, C1q signals with WOX1 for inducing microvillus formation in clusters in between cells, followed by fragmentation. This results in thousands of fragments containing WOX1 and amyloid beta adhered on the extracellular matrix. The induction is blocked by dominant negative WOX1, anti‐C1q receptor antibody, and inhibitors for G protein/Rho/ROCK signaling and metalloproteinases. Meanwhile, cytosolic WOX1 undergoes self‐aggregation, leading to apoptosis. Aggregated WOX1 is shown with amyloid beta plaques in the hippocampus of patients with Alzheimer's disease, suggesting a role of C1q/WOX1 signaling in contributing to neurodegeneration. (Supported in part by NSC and NHRI, Taiwan, and DoD, USA)