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SIN‐1 cause downregulation of sirtuin expression in mouse astrocytes
Author(s) -
Xie Qian Reuben,
Shao Jiaxiang,
Ying Weihai,
Xia Weiliang
Publication year - 2010
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.24.1_supplement.708.13
Subject(s) - sirt2 , resveratrol , sirt3 , sirtuin , sirt6 , inflammation , oxidative stress , sirtuin 1 , downregulation and upregulation , microbiology and biotechnology , biology , chemistry , pharmacology , acetylation , immunology , biochemistry , gene
Astrocytes play essential roles in mediating inflammation in ischemic brain diseases. Sirtuins are important proteins regulating multiple biological processes including aging, inflammation and survival. The expression and function of sirtuins in astrocytes have not been reported. In this study, we use primary cultures of mouse astrocytes to study the expression of sirtuins, and how their levels respond in various conditions including oxidative stress induced by 3‐morpholinosydnonimine (SIN‐1), resveratrol, sirtinol, or pre‐treated with resveratrol or sirtinol followed by SIN‐1. Mouse astrocytes express all seven forms of sirtuins with sirt2, sirt3, sirt7 being the highest expressed. SIN‐1 (0.2–1 mM) profoundly down‐regulated the expression of all forms of sirtuins. Sirtinol (50–200 uM) significantly down‐regulated the expression of all forms of sirtuins except sirt3. Resveratrol (50–150 uM) did not significantly affect the expression of sirtuins. However, pretreatment of reseveratrol (100 uM) but not sirtinol attenuated the effect of SIN‐1 in downregulating the expression of sirtuins. Our findings suggest oxidative stress and the modulation of sirtuins expression might be closely linked. (Supported by “Rising Star” Grant from Science and Technology Commission of Shanghai and Young Investigator Grant from National Natural Science Foundation of China to W. X.)