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Characterization of the orphan human (C‐X‐C) chemokine receptor type 7 receptor (CXCL7) on T lymphocyte cells
Author(s) -
Vu John Peter,
Germano Patrizia,
Lu Yuxin,
Pisegna Joseph
Publication year - 2010
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.24.1_supplement.705.6
Subject(s) - chemokine receptor , g protein coupled receptor , receptor , calcitonin gene related peptide , c c chemokine receptor type 6 , microbiology and biotechnology , ccr3 , cxc chemokine receptors , orphan receptor , ccl25 , jurkat cells , cc chemokine receptors , chemistry , cxcl14 , ccr1 , chemokine , biology , biochemistry , immunology , t cell , immune system , neuropeptide , gene , transcription factor
Chemokines function as the main regulators of leukocyte trafficking under inflammatory conditions. Chemokine receptors have a 7 transmembrane structure and belong to the G protein‐coupled receptor subfamily. G‐Protein coupled receptors (GPCRs) mediate cellular responses to a diverse array of signaling molecules such as neurotransmitters, phosophilipds, and peptide hormones. Receptors coupled to G proteins form the largest known family of surface receptors, however despite the identification of hundreds human GPCRs, more than half are termed orphan. Recently, a CXCL7 (RDC1) has been cloned as an orphan GPCR receptor that shares homology with chemokine receptors. Initially, RDC1 was reported as a receptor vasoactive intestinal peptide, however this observation was dismissed when it was shown that RDC1 could not account for calcitonin gene‐related peptide (CGRP) and adrenomedullin (ADM) binding. Here we describe the characterization of the RDC1 receptor expression on T lymphocyte cells. Jurkat cells were stably transfected to overexpress the RDC1 receptor. Gene expression profiles were studied with transfected RDC‐1 and untransfected Jurkat cells 2 and 24 hour conditions in response to either anti‐CD3, CGRP, and ADM with and with out the partial CGRP receptor antagonist CGRP8‐37. Intracellular calcium signaling responded to CGRP, ADM, and slight inhibitory response to CGRP (8–37) . Based upon calcium signaling studies, the cells showed a response to anti‐CD3, CGRP and ADM and showed a slight response to CGRP (8–37) . These results will permit a more detailed determination as to the localiazation and physiological function of this novel receptor.