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GDP‐mannose transporter oligomer formation in the filamentous fungus Aspergillus nidulans
Author(s) -
Johnson Laura Raquel,
JacksonHayes Loretta,
Hill Terry W.,
Loprete Darlene M.
Publication year - 2010
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.24.1_supplement.695.7
Subject(s) - aspergillus nidulans , golgi apparatus , mannose , oligomer , saccharomyces cerevisiae , microbiology and biotechnology , cytosol , subcellular localization , biochemistry , chemistry , biology , endoplasmic reticulum , yeast , gene , enzyme , organic chemistry , mutant
GDP‐mannose transporters (Gmt) carry nucleotide sugars from the cytosol across the Golgi apparatus membrane in various eukaryotic organisms. Some fungal species like Saccharomyces cerevisiae express a single gmt , while others including A. nidulans express two ( gmtA and gmtB ). GmtA and GmtB display a punctate pattern of distribution indicative of localization within the Golgi apparatus. Gmt in S. cerevisiae localizes to the Golgi as well, and proper localization is dependent on oligomerization. Here we report progress on determining if GmtA and GmtB form oligomers and the preferred combinations of GmtA and GmtB for Golgi localization. In addition, C ‐ and N ‐ terminal truncated versions of GmtA and GmtB were created in order to designate regions responsible for Golgi localization and possible oligomer formation.