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Mapping of peptides and proteins in Leech (Hirudo medicinalis) by Mass Spectrometry Imaging
Author(s) -
Sistla Srinivas,
Bruand Jocelyne,
Ghassemian Majid,
Bafna Vineet,
Gaasterland Terry,
Salzet Michel,
Macagno Eduardo
Publication year - 2010
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.24.1_supplement.670.12
Subject(s) - hirudo medicinalis , leech , peptide , biology , mass spectrometry , tandem mass spectrometry , protease , peptide sequence , chemistry , computational biology , biochemistry , microbiology and biotechnology , enzyme , chromatography , world wide web , computer science , gene
Mass Spectrometry imaging (MSI) can be used to obtain the expression profiles of proteins and peptides in various biological tissues under different experimental conditions. In this study, we employed MSI to map the temporal and spatial distributions of many individual molecules in the range of m/z from 1kD to ~10kD in the embryo of the medicinal leech ( Hirudo medicinalis ). Distributions with specific characteristics such as selective expression in the central nervous system or localized expression in certain areas of the body wall were selected for further analysis. For sequence identification, leech protein/peptide embryo extracts were prepared using different conditions and run on LC ESI QTOF and on LC MALDI instruments. Peaks corresponding to masses associated with selected distributions were sequenced using database dependent and denovo methods. An interesting example of peptides identified in this manner are proteolytic fragments from histones, protease inhibitors like cystatins and other proteins that have a role in the innate immune system of lower invertebrates. First and second author contributed equally Supported by NSF Grant DBI‐0852081