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MicroRNA Expression Analysis of Blood Samples from African Green NHP Exposed to Yersinia Pestis
Author(s) -
Jibitu Meskerem,
Miller Stacy Ann,
Waddy Edward Byron,
Muhie Seid,
Hammamieh Rasha,
Jett Marti
Publication year - 2010
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.24.1_supplement.666.1
Subject(s) - yersinia pestis , biology , microrna , host (biology) , dna microarray , microarray , immune system , microbiology and biotechnology , virology , gene expression , gene , immunology , genetics , virulence
Yersinia pestis , the causative agent of plague, is known to develop strategies to overcome the host immune mechanisms and survive in the host. The molecular changes induced by Y. pestis in the host are not well delineated. Here, we examined the early events triggered after the intracellular infection of Y. pestis in NHPs by analyzing the host microRNA profiles using microRNA microarrays. Monkey Blood samples treated with Yersinia pestis and sham controls were collected at room temperature using the QIAGEN™ protocol. RNA samples were isolated and assessed using the Bioanalyzer. SuperArray miRNA PCR Array were used to hybridize the isolated samples. Data were analyzed to identify miRNAs that were differentially expressed in infected NHPs. Some of the identified miRNAs are known to target transcription factors, molecular transport and apoptosis.