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The effect of bacterial challenge on ferritin regulation in Aedes aegypti
Author(s) -
Geiser Dawn L.,
Zhou Guoli,
Kohlhepp Edwin A.,
Mayo Jonathan J.,
Winzerling Joy J.
Publication year - 2010
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.24.1_supplement.658.2
Subject(s) - ferritin , aedes aegypti , midgut , biology , secretion , microbiology and biotechnology , biochemistry , botany , larva
In mammals, ferritin is a cytoplasmic iron storage protein made up of 24 heavy and light chain subunits that can store up to 4500 iron atoms; as wells as an acute phase protein that is secreted upon inflammation. Here we characterize the message and protein expression profiles of Aedes aegypti (yellow fever mosquito) ferritin heavy chain (HCH) and light chain homologue (LCH) subunits in response to iron and bacterial challenge. Mosquito experiments show an increase in midgut HCH and LCH message and protein expression 24 h post‐blood meal (PBM) and decrease 72 h PBM. Fat body and ovary HCH and LCH message and protein expression showed a time‐dependent increase PBM. Heat‐killed bacteria (HKB) had little effect on mosquito tissue ferritin expression. Iron regulatory protein (IRP) binding activity for the iron responsive element (IRE) increased over time in ovary PBM. Midgut and fat body binding activity increased 24 h PBM with a decrease 72 h PBM. Mosquitoes treated with HKB showed no change in IRP/IRE binding activity compared to naïve animals. Cell studies support in vivo results. HCH and LCH message and protein expression reveal a time‐dependent, synergistic up‐regulation of expression in CCL‐125 cells treated with iron and HKB than iron alone. There was also a time‐dependent increase in secreted ferritin as well as decrease IRP/IRE binding activity in cells treated with iron and HKB. Research support from NIH ( GM056812 ), the Agricultural Experiment Station and CALS at the University of Arizona.