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The design and characterization of an RNA nanoring for use in RNA interference drug delivery
Author(s) -
Grabow Wade,
Severcan Isil,
Chworos Arkadiusz,
Shapiro Bruce,
Luc Jaeger
Publication year - 2010
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.24.1_supplement.655.2
Subject(s) - rna interference , rna , circular rna , nanoring , rational design , nanotechnology , chemistry , computational biology , biophysics , biology , gene , materials science , biochemistry
While RNA interference (RNAi) offers tremendous promise as a selective means to silence gene expression, novel packaging and delivery methods are needed to improve its overall therapeutic effectiveness. The following RNA nanoring was previously designed as a simple means to package and deliver RNAi. Characterization of the RNA nanoring and the contribution of the RNAI/RNAII inverse interaction responsible for its specific assembly were carried out through the rational design of monomers possessing helical stems with altered degrees of stability, twist, and rigidity. Experimental analysis of the different monomer assemblies confirms that the geometry of the loop‐loop interaction is the dominant factor in the formation of RNA nanorings. Subsequent studies on one specific RNA nanoring design have demonstrated a promising level of resistance toward ribonuclease degradation and confirm its potential as a platform for RNAi drug delivery. This work was funded by the National Institutes of Health (RO1 GM079604‐01 ) to Luc Jaeger.