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The role of Toll‐like receptor 3 in apoptosis of bewo cells hepatitis B virus induced
Author(s) -
Wang Suping,
Li Xiaoyan,
Song Xiuxia,
Feng Liping,
Qu Yiqun
Publication year - 2010
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.24.1_supplement.650.3
Subject(s) - apoptosis , flow cytometry , hepatitis b virus , tlr3 , cccdna , biology , microbiology and biotechnology , virus , virology , immunology , toll like receptor , immune system , hbsag , innate immune system , biochemistry
Objective The aim of this study was to explore whether HBVcan replicate in bewo cells, whether HBV can induce apoptosis of bewo cells, and the role TLR3 may play in apoptosis induced by HBV. Methods PCR was used to detect HBV cccDNA expression of bewo cells cocultured with HBV positive serum. Flow cytometry, tarnsefarse‐mediated dUTP nick end‐labeling were used to detect apoptosis of bewo cells. Flow cytometry was conducted to measure TLR3 protein. Results HBV cccDNA can be detected in bewo cells co‐cultured with HBV DNA positive serum for 48 hours. when bewo cells were cocultured with 100μl HBV serum for 24h or 48h, early apoptotic rates, total apoptotic rates and apoptotic indexes were all statistically higher than that in the separate cotemporaneous group (P < 0.001). After PolyI:C prestimulation, early apoptotic rates and total apoptotic rates of the bewo cells co‐cultured with HBV serum for 24h or 48h were significantly decreased(P < 0.05); TLR3 protein's mean fluorescence intensity was statistically higher than that in no‐PolyI:C stimulated group(P=0.02). Conclusion Bewo cells which hepatitis B virus can replicate in may be a good model for HBV infection experiments. TLR3 may mediate the apoptosis of bewo induced by HBV.The activation of TLR3 by PolyI: C may inhibit apoptosis of bewo induced by HBV.